genetic modification

Fighting Malaria With Gene-Drive Technology | Earth Wise

June 8, 2020 By EarthWise Leave a Comment

Malaria continues to be a major health hazard throughout the tropical and subtropical regions of the world. There were 228 million cases of malaria in 2018 and over 400,000 deaths.

Malaria is a mosquito-borne infectious disease spread by 40 of the world’s 3,500 mosquito species. So, efforts to control mosquito populations are the primary strategy to eradicate malaria.

A team led by Imperial College London has created a genetic modification that distorts the sex ratio of a population of Anopheles gambiae mosquitoes using “gene drive” technology. The modification works by using a DNA-cutting enzyme to destroy the X chromosome during the production of sperm, which leads to predominantly male offspring, since females require two X chromosomes. The modification is coupled to a gene drive to allow it to spread through a population in a very effective way. A gene drive is a genetic engineering technology that propagates a particular modification by assuring that a specific form of a gene (or allele) will be transmitted with far more than the natural 50% probability.

The result of this is that mosquitoes produce more male offspring, eventually leading to no females being born and a total collapse in the population. The mosquitoes studied are the main malaria vector in sub-Saharan Africa. The hope is that mosquitoes carrying a sex-distorter gene drive would be released in the future, spreading the male bias with local malaria-carrying populations and causing them to collapse. Only female mosquitoes bite and take blood meals. If the gene drive technology works in the field, it could be a game-changer in the fight to eliminate malaria.

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Malaria mosquitoes eliminated in lab by creating all-male populations

Photo, posted June 20, 2014, courtesy of Flickr.

Earth Wise is a production of WAMC Northeast Public Radio.

Engineering Mosquitoes | Earth Wise

February 21, 2020 By EarthWise Leave a Comment

Tropical regions grapple with the spread of diseases – such as dengue, yellow fever, zika, chikungunya, and malaria – by mosquitoes. A fairly successful strategy has been the Sterile Insect Technique, which is essentially insect birth control. The process involves rearing large quantities of sterilized male mosquitoes in dedicated facilities, and then releasing them to mate with females in the wild. As they do not produce any offspring, the insect population declines over time.

A problem with this approach is that while mosquitoes create health problems for people, they also play important roles in various ecosystems, such as providing food for bats and other animals. Eliminating mosquito populations on a large scale can trigger major changes in ecosystems.

Recently, an international team of scientists has synthetically engineered mosquitoes that halt the transmission of the dengue virus. They genetically engineered mosquitoes with an antibody “cargo” that gets expressed in the female mosquitoes that spread the dengue virus. Once the female mosquito takes in blood, the antibody is activated which hinders the replication of the virus and prevents its dissemination throughout the mosquito, thereby preventing its transmission to humans. Essentially, what the researchers have done is transfer genes from the human immune system to confer immunity to mosquitoes. The researchers are testing methods to neutralize mosquitoes against other viruses they spread.

This opens up a whole new approach to interrupt mosquito-borne human diseases. Mosquitoes are among the deadliest killers on the planet because they are the messengers that transmit deadly diseases. Until now, the only real solution has been to kill the messenger. The new approach may be a better way to deal with a serious problem.

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Mosquitoes engineered to repel dengue virus

Photo, posted June 20, 2014, courtesy of Flickr.

Earth Wise is a production of WAMC Northeast Public Radio.

Hacking Photosynthesis

February 25, 2019 By EarthWise Leave a Comment

There is an enzyme known as RuBisCo that is involved in carbon fixation, the process plants use to convert carbon dioxide into sugar molecules. The RuBisCo molecule is inside the leaves of most plants and is probably the most abundant protein in the world.

RuBisCo picks up carbon dioxide from the air and uses energy from the sun to turn the carbon into sugar molecules. This process of photosynthesis is pretty much the foundation of life on Earth.

Wonderful as it is, the process is not perfect. RuBisCo is not very selective in grabbing molecules from the air. It picks up oxygen as well as CO₂ and it produces a toxic compound when it does that.

Plants operate a whole other complicated chemical process to deal with this toxic byproduct and uses up a lot of energy along the way, leaving less energy for making leaves or food that we can eat.

A research program at the University of Illinois called Realizing Increased Photosynthetic Efficiency (or RIPE) has been trying to correct this problem; they have been trying to hack photosynthesis. And they may well have succeeded.

Using genetic modification on tobacco plants, they have shut down the existing detoxification process and set up a much more efficient new one. The result is super plants that grow faster and up to 40% bigger.

The next step is to get it to work on plants that people actually rely upon for food, like tomatoes, soybeans and black-eyed peas (which are a staple food crop in sub-Saharan Africa where food is scarce.)

It will be years before we know if the process can really produce more food and be safe, but it may end up leading to a major increase in crop productivity.

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Scientists Have ‘Hacked Photosynthesis’ In Search Of More Productive Crops

Photo, posted June 10, 2013, courtesy of Boon Hong Seto via Flickr.

Earth Wise is a production of WAMC Northeast Public Radio.